DNA Molecular Weight Markers

 

DNA MW 0.1-14kb (Lambda - BstE II Digest)

Description: 14 fragments ranged from 117bp to 14,140bp suitable for use as quantitative molecular weight marker for agarose gel electrophoresis.

Concentration: 200 µg/µl

Recommended volume to load - 5 µl, equivalent to 1 µl of DNA

Storage: 2 months at +4 °C, 1 year at -20 °C.

Shipment at ambient temperature.

5 µl (1 µg) of DNA MW 0.1-14kb, 1% agarose, stained with ethidium bromide:

Marker Composition, 5µl:
Size, bp Mass, ng
14,140 290
7,242 150
6,369 130
4,822 100
4,324 90
3,675 75
2,323 50
1,929 40
1,371 30
1,264 25
702 15
224* 5
117* 2

*Bands corresponding to 224 bp and 117 bp are minor and may not be visible.

NOTE: Fragment 14,140 bp is composed of fragments 8,454 bp and 5,686 bp with cohesive ends that may be  separated by heating at 60 °C for 3 minutes. Higher temperature may cause DNA denaturation.

 
 

 

Cat.#

Pack Size

Price

B1001

50 lanes, 50 µg, 250 µl

£40

B1002

200 lanes, 0.2 mg, 1 ml

£80

B1003

1000 lanes, 1 mg, 5x1ml

£220

 


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