PCR Buffers (A to W)

 

Choosing the right buffer for a particular DNA polymerase and specific application plays a key role in PCR optimisation. Poor performance of enzymes, low or no yield are consequences of employing the wrong buffer, in most cases. We offer both individual and sets of 10 PCR buffers which have emerged during history of PCR for screening purposes. Buffers are prepared using DNase- and RNase-free water. The pH was measured at 25 C. All buffers were assayed in reactions with a number of commercially available DNA polymerases. Detergent-free buffers suitable for DHPLC are highlighted.

All buffers can be supplemented with MgCl2, MgSO4, or Mg-Acetate upon request.

Please contact us if you need any advice on optimum buffer composition for a PCR procedure you experience difficulties with.

 

 Individual 10 PCR buffers:

 

Name

Composition, 10

pH

Pack size

Cat #

Price

A

375mM Tris-Cl, 200mM (NH4)2SO4, 0.1% Tween-20

8.8

5 1.2 ml

S1102

20

B

670mM Tris-Cl, 150mM (NH4)2SO4, 0.1% Tween-20

8.8

5 1.2 ml

S1104

20

C

1.0M Tris-Cl, 200mM (NH4)2SO4, 0.1% Tween-20

8.4

5 1.2 ml

S1106

20

D

600mM Tris-Cl, 170mM (NH4)2SO4, 0.1% Tween-20

9.0

5 1.2 ml

S1108

20

E

500mM Tris-Cl, 200mM (NH4)2SO4

8.4

5 1.2 ml

S1110

20

F

500mM Tricin-KOH, 170mM (NH4)2SO4, 0.1% Tween-20

8.7

5 1.2 ml

S1112

20

G

500mM Tricin-KOH, 150mM (NH4)2SO4

8.7

5 1.2 ml

S1114

20

H

500mM Tris-Cl, 500mM KCl

8.4

5 1.2 ml

S1116

20

I

400mM Tris-Cl, 500mM NaCl

7.5

5 1.2 ml

S1118

20

J

500mM Tricin-KOH, 450mM KAcetate, 5% DMSO, 40% glycerol

8.7

5 1.2 ml

S1120

20

K

500mM Tris-Cl

9.5

5 1.2 ml

S1122

20

L

100mM Tris-Cl, 50mM DTT

7.5

5 1.2 ml

S1124

20

M

100mM Tris-Cl, 100mM KCl

8.3

5 1.2 ml

S1126

20

N

100mM Tris-Cl, 500mM KCl, 1% Triton X-100

8.9

5 1.2 ml

S1128

20

O

100mM Tris-Cl, 500mM KCl

8.0

5 1.2 ml

S1130

20

P

400mM Tris-Cl, 650mM K-Glutamate, 1% Triton X-100

8.7

5 1.2 ml

S1132

20

Q

400mM Tris-Cl, 650mM K-Acetate, 1% Triton X-100

8.7

5 1.2 ml

S1134

20

R

600mM Tris-Cl, 60mM (NH4)2SO4, 100mM KCl

8.3

5 1.2 ml

S1136

20

S

500mM Tris-Cl, 50mM (NH4)2SO4, 100mM KCl

9.0

5 1.2 ml

S1138

20

T

200mM Tris-Cl, 100mM (NH4)2SO4, 100mM KCl, 1% Triton X-100

8.9

5 1.2 ml

S1140

20

U

1M Tris-Cl, 100mM (NH4)2SO4, 100mM KCl, 1% Triton X-100

8.7

5 1.2 ml

S1142

20

V

200mM Tris-Cl, 100mM (NH4)2SO4, 100mM KCl, 1% Triton X-100, 1mg/ml BSA

8.7

5 1.2 ml

S1144

20

W

100mM Tris-Cl, 500mM KCl, 1% Triton X-100

8.3

5 1.2 ml

S1146

20

 

 

 

Sets of PCR buffers

 

 

10 PCR Buffers Set 1 (no detergents)

 

Description:

Set of 10 PCR buffers E, G, H, I, J, K, L, M, O, R, S

 

Content:

11 1.2 ml of each buffer

1 1.2 ml of 50mM MgCl2

1 1.2 ml of 50mM MgSO4

1 1.2 ml of 50mM Mg acetate

Protocol

Storage:

24 months at -20 C in a constant temperature freezer

Catalogue #

S2101

Price

60

 

 

10 PCR Buffers Set 2 (with detergents)

 

Description:

Set of 10 PCR buffers A, B, C, D, F, N, P, Q, T,U, V, W

 

Content:

12 1.2 ml of each buffer

1 1.2 ml of 50mM MgCl2

1 1.2 ml of 50mM MgSO4

1 1.2 ml of 50mM Mg acetate

Protocol

Storage:

24 months at -20 C in a constant temperature freezer

Catalogue #

S2102

Price

70

 

 

10 PCR Buffers Set A-W

 

Description:

Set of 10 PCR buffers A to W

 

Content:

23 1.2 ml of each buffer

1 1.2 ml of 50mM MgCl2

1 1.2 ml of 50mM MgSO4

1 1.2 ml of 50mM Mg acetate

Protocol

Storage:

24 months at -20 C in a constant temperature freezer

Catalogue #

S2101

Price

110

 

 

Associated products:

 

DNA polymerases

dNTPs

PCR additives

 

 

 

 

 

PCR additives

 

 

2 DNA Softner

 

Description: Universal additive to any PCR mix. Proprietary optimised composition of several additives. Helps in all difficult cases. Improves specificity, sensitivity and yield of reaction as well as performance of polymerases. Action is similar to melting of DNA regions with complex secondary structure and making DNA more accessible to polymerase. Tested at up to 76% of GC-, and 68% of AT-rich sequences.

Applications: PCR on GC- or AT-rich templates including DNA sequencing, cloning and detection.

Shipping Conditions: Any temperature equal or below ambient

Storage Conditions: +4 C for short time (up to 2 weeks), -20 C for months

Note: If precipitate heat at 65 C for 5 min and vortex until crystals disappear.

 

 

Cat.#

Pack Size

Price

 

 

S1150

Sample 0.25 ml

Free of charge

 

 

S1151

1.2 ml

30

 

 

S1152

5 1.2 ml

80

 


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