|
PCR Buffers (A to W)
Choosing the right buffer for a
particular DNA polymerase and specific application plays a key role in PCR
optimisation. Poor performance of enzymes, low or no yield are consequences of
employing the wrong buffer, in most cases. We offer both individual and sets of
10 × PCR buffers which have emerged during history of PCR for screening
purposes. Buffers are prepared using DNase- and RNase-free water. The pH was
measured at 25 ºC. All buffers were assayed in reactions with a number of
commercially available DNA polymerases. Detergent-free buffers suitable for
DHPLC are highlighted.
All buffers can be supplemented with
MgCl2, MgSO4, or Mg-Acetate upon request.
Please
contact us if you need any advice on optimum buffer
composition for a PCR procedure you experience difficulties with.
Individual
10 × PCR buffers:
|
Name |
Composition, 10 × |
pH |
Pack size |
Cat # |
Price |
|
A |
375mM Tris-Cl, 200mM (NH4)2SO4,
0.1% Tween-20 |
8.8 |
5 × 1.2 ml |
S1102 |
£20 |
|
B |
670mM Tris-Cl, 150mM (NH4)2SO4,
0.1% Tween-20 |
8.8 |
5 × 1.2 ml |
S1104 |
£20 |
|
C |
1.0M Tris-Cl, 200mM (NH4)2SO4,
0.1% Tween-20 |
8.4 |
5 × 1.2 ml |
S1106 |
£20 |
|
D |
600mM Tris-Cl, 170mM (NH4)2SO4,
0.1% Tween-20 |
9.0 |
5 × 1.2 ml |
S1108 |
£20 |
|
E |
500mM Tris-Cl, 200mM (NH4)2SO4
|
8.4 |
5 × 1.2 ml |
S1110 |
£20 |
|
F |
500mM Tricin-KOH, 170mM
(NH4)2SO4, 0.1% Tween-20 |
8.7 |
5 × 1.2 ml |
S1112 |
£20 |
|
G |
500mM Tricin-KOH, 150mM
(NH4)2SO4 |
8.7 |
5 × 1.2 ml |
S1114 |
£20 |
|
H |
500mM Tris-Cl, 500mM KCl |
8.4 |
5 × 1.2 ml |
S1116 |
£20 |
|
I |
400mM Tris-Cl, 500mM NaCl |
7.5 |
5 × 1.2 ml |
S1118 |
£20 |
|
J |
500mM Tricin-KOH, 450mM
KAcetate, 5% DMSO, 40% glycerol |
8.7 |
5 × 1.2 ml |
S1120 |
£20 |
|
K |
500mM Tris-Cl |
9.5 |
5 × 1.2 ml |
S1122 |
£20 |
|
L |
100mM Tris-Cl, 50mM DTT |
7.5 |
5 × 1.2 ml |
S1124 |
£20 |
|
M |
100mM Tris-Cl, 100mM KCl |
8.3 |
5 × 1.2 ml |
S1126 |
£20 |
|
N |
100mM Tris-Cl, 500mM KCl,
1% Triton X-100 |
8.9 |
5 × 1.2 ml |
S1128 |
£20 |
|
O |
100mM Tris-Cl, 500mM KCl |
8.0 |
5 × 1.2 ml |
S1130 |
£20 |
|
P |
400mM Tris-Cl, 650mM
K-Glutamate, 1% Triton X-100 |
8.7 |
5 × 1.2 ml |
S1132 |
£20 |
|
Q |
400mM Tris-Cl, 650mM
K-Acetate, 1% Triton X-100 |
8.7 |
5 × 1.2 ml |
S1134 |
£20 |
|
R |
600mM Tris-Cl, 60mM (NH4)2SO4,
100mM KCl |
8.3 |
5 × 1.2 ml |
S1136 |
£20 |
|
S |
500mM Tris-Cl, 50mM (NH4)2SO4,
100mM KCl |
9.0 |
5 × 1.2 ml |
S1138 |
£20 |
|
T |
200mM Tris-Cl, 100mM (NH4)2SO4,
100mM KCl, 1% Triton X-100 |
8.9 |
5 × 1.2 ml |
S1140 |
£20 |
|
U |
1M Tris-Cl, 100mM (NH4)2SO4,
100mM KCl, 1% Triton X-100 |
8.7 |
5 × 1.2 ml |
S1142 |
£20 |
|
V |
200mM Tris-Cl, 100mM (NH4)2SO4,
100mM KCl, 1% Triton X-100, 1mg/ml BSA |
8.7 |
5 × 1.2 ml |
S1144 |
£20 |
|
W |
100mM Tris-Cl, 500mM KCl,
1% Triton X-100 |
8.3 |
5 × 1.2 ml |
S1146 |
£20 |
Sets
of PCR buffers
|
10 × PCR Buffers Set 1 (no
detergents)
|
|
Description: |
Set of 10 × PCR buffers E, G,
H, I, J, K, L, M, O, R, S
|
|
Content: |
11 × 1.2 ml of each buffer
1 × 1.2 ml of 50mM MgCl2
1 × 1.2 ml of 50mM MgSO4
1 × 1.2 ml of 50mM Mg acetate
Protocol |
|
Storage: |
24 months at -20 ºC in a
constant temperature freezer |
|
Catalogue # |
S2101 |
|
Price |
£60 |
|
10 × PCR Buffers Set 2 (with
detergents)
|
|
Description: |
Set of 10 × PCR buffers A, B,
C, D, F, N, P, Q, T,U, V, W
|
|
Content: |
12 × 1.2 ml of each buffer
1 × 1.2 ml of 50mM MgCl2
1 × 1.2 ml of 50mM MgSO4
1 × 1.2 ml of 50mM Mg acetate
Protocol |
|
Storage: |
24 months at -20 ºC in a
constant temperature freezer |
|
Catalogue # |
S2102 |
|
Price |
£70 |
|
10 × PCR Buffers Set A-W
|
|
Description: |
Set of 10 × PCR buffers A to W
|
|
Content: |
23 × 1.2 ml of each buffer
1 × 1.2 ml of 50mM MgCl2
1 × 1.2 ml of 50mM MgSO4
1 × 1.2 ml of 50mM Mg acetate
Protocol |
|
Storage: |
24 months at -20 ºC in a
constant temperature freezer |
|
Catalogue # |
S2101 |
|
Price |
£110 |
Associated products:
DNA
polymerases
dNTPs
PCR
additives
PCR
additives
|
2 × DNA Softner
|
|
Description:
Universal additive to any
PCR mix. Proprietary optimised composition of several additives. Helps
in all difficult cases. Improves specificity, sensitivity and yield of
reaction as well as performance of polymerases. Action is similar to
“melting” of DNA regions with complex secondary structure and making DNA
more accessible to polymerase. Tested at up to 76% of GC-, and 68% of
AT-rich sequences. |
|
Applications:
PCR on GC- or AT-rich
templates including DNA sequencing, cloning and detection. |
|
Shipping
Conditions: Any temperature
equal or below ambient |
|
Storage
Conditions: +4 ºC for short
time (up to 2 weeks), -20 ºC for months |
|
Note:
If precipitate – heat at 65 ºC for 5 min and vortex until crystals
disappear.
|
|
|
Cat.# |
Pack Size |
Price |
|
|
|
S1150 |
Sample 0.25 ml |
Free of charge |
|
|
|
S1151 |
1.2 ml |
£30 |
|
|
|
S1152 |
5 × 1.2 ml |
£80 |
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