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Random DNA mutagenesis
We can introduce random mutations to the gene supplied
by a customer.
Mutated DNA will be then cloned into a vector of choice to obtain a library of
the mutated DNA
- Mutation frequency can be set to a desired value
within 1-20 mutations per kb
- Number of clones 106-108
- Mutations can be restricted to a desired region of
a gene
Customer provides:
Vector/plasmid containing DNA to be mutated
Map of that plasmid and sequence of the target DNA
Instructions on desired mutation frequency and number of clones
Our service includes:
DNA mutagenesis specified
Cloning of the mutations library into a vector of choice
Duration:
106 clones: 3- 4 weeks
108 clones: 7-8 weeks
Customer receives:
Report with mutation library characteristics and sequence data for 10
randomly selected clones
E. coli stock containing a mixture of clones with unique random mutations
mutations
Price guide:
106 clones: £2900
108 clones: £3700
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