siRNA functional assay

Be at the cutting edge of transcriptomics research with our new siRNA-target match validation service!

This unique research service allows the customer to estimate the functional efficacy of one or several siRNA targets and to choose the best one for following in vivo
experiments. Our service includes quantitative functional validation and efficacy measurements for any siRNA target sequence.

Customer provides:

The sequence of the siRNA to be tested (19 – 29 bases corresponding to the mRNA strand). Such sequence data can be obtained empirically using software available online.
 

Our service includes:

• Oligonucleotide synthesis and cloning of the indicated siRNA target sequence into a proprietary reporter plasmid.
  Simultaneous preparation of the effector plasmid to express doublestranded siRNA directed to the same target carried by the reporter plasmids
• Effector plasmid and each of the reporter plasmids will be cotransfected into HEK293 human cells in duplicates. Reporter activity will be measured 48 h post transfection. Functional efficacy of the given target will be calculated as the mean reporter silencing for upstream and downstream target positions relating to the reporter coding sequence.
• Duration: 15-20 working days
   

Customer receives:

• Calculated siRNA quantitative functional efficacy
• Detailed report on experiments and data obtained

Price guide:

Basic price per siRNA target sequence £600