Oligonucleotides Synthesis Service

15-150 nt-long, any scale and modifications

Oligonucleotides synthesis:

Quality: Reverse phase purification of oligonucleotides as standard procedure

Delivery: 3, 5 and 7 working days for unmodified, single-, and double- labelled oligonucleotides respectively.

Ordering via: email (sales@york-bio.com), fax (01904 567 621), mail, or online order form

Price starts from 30p/base (10 nmole scale).

Discounts: 10% off the price for >10 oligos/order.

Report: Complete documentation is enclosed.

Your Choice: Let us know if you would like to receive oligonucleotides dissolved in water or TE at working concentration.

Standard Oligonucleotides:

Synthesis Scale	Number of Bases	Yield (20-mer)	Purification	Price/base, (£)
10 nmole	15 - 35	2 OD	RPC*	0.40
40 nmole	15 - 80	4 OD	RPC*	0.50
40 nmole	80-150	2 OD	HPLC**	1.20
0.2 µmole	15 - 80	15 OD	RPC*	0.80
1.0 µmole	15 - 80	50 OD	Gel-filtration	1.80
10 µmole	15-50	400 OD	HPLC, £250	15.0
15 µmole	15-50	600 OD	HPLC, £350	18.0

Purification: *RPC (reverse phase chromatography) and gel-filtration are free of charge. HPLC** purification for 1 µmole and below - £20.

Prices on Labelled Oligonucleotides (15-80 bases), including HPLC purification:

Label or Modification	10 nmole	40 nmole	0.2 µmole	1.0 µmole	Unit
Standard DNA	£0.40	£0.50	£0.80	£1.80	base
Phosphorothioate	£5.80	£7.50	£8.50	£12.00	base
5’-Amino	£50	£60	£75	£85	oligo
3’-Amino	£50	£60	£75	£85	oligo
5’-Phosphorylation	£50	£60	£75	£85	oligo
3’-Phosphorylation	£40	£50	£80	£100	oligo
Biotin	£50	£60	£75	£85	oligo
FITC	£40	£60	£75	£85	oligo
TRITC	£40	£60	£75	£85	oligo
6-Fam	£60	£80	£95	£120	oligo
Tet	£60	£80	£95	£120	oligo
Hex	£60	£80	£95	£120	oligo
6-Rox	£80	£90	£110	£160	oligo
6-Joe	£80	£90	£110	£160	oligo
6-Tamra	£80	£90	£110	£160	oligo
Texas Red	£80	£90	£110	£160	oligo
Cy5	£80	£90	n/a	n/a	oligo
Cy3	£80	£90	n/a	n/a	oligo
DNP (Dinitrophenyl)	£60	£80	£95	£120	oligo
Dabsyl	£60	£80	£95	£120	oligo
Dual labelled oligonucleotides, including 2×HPLC: Possible dyes at 5’: 6-Fam, Hex, Tet Possible dyes at 3’: Tamra, Rox, Joe	£240	£300	£400	£800	oligo
Guaranteed yield	1.0 OD	2.0 OD	5.0 OD	15.0 OD	oligo

For oligos shorter than 15 or longer than 80 bases, please enquire.

Oligonucleotides order form:

Please provide your contact details (*- required fields):

*First Name

*Last Name

Title

* Phone

FAX

*E-mail

Please proceed to ordering unmodified or /and modified/labelled oligonucleotides. Prices, terms and conditions are in the table above.

NOTE: use following abbreviation when ordering oligonucleotides containing degenerate bases:

· A+G=R	· G+T=K	· A+T+C=H	· G+A+T=D	· Deoxyinosine =I
· C+T=Y	· G+C=S	· G+T+C=B	· A+C+T+G=N	· Deoxyuridine =U
· A+C=M	· A+T=W	· G+A+C=V

UNMODIFIED OLIGONUCLEOTIDES ORDER FORM:

OLIGO NAME

5'-SEQUENCE- 3'

SCALE
PURIFICATION

MODIFIED AND LABELLED OLIGONUCLEOTIDES ORDER FORM (HPLC purified only):

MODIFICATION/LABELLING

OLIGO NAME

5'-SEQUENCE- 3'

SCALE

5'-end

3'-end

internal

Notes or comments on oligonucleotides modification or labelling (use table above for options):

*Purchase Order #:

Account Name if known:

SHIPPING ADDRESS:

*Organization

*Department/Address

Address (cont.)

*City

*State/County

*Zip/Postal Code

*Country

* - required fields

BILLING address if different from above:

Organization

Street Address

Address (cont.)

City

State/County

Zip/Postal Code

Country

Phone

FAX

E-mail

VAT status:

Payable
Exempt (please fax or send the certificate)

DO NOT FORGET TO PRINT THIS FORM FOR YOUR RECORD!

                                                    Yorkshire Bioscience Ltd, Biocentre, York Science Park, Heslington, York YO10 5NY, UK
                                                    Tel: +44 (0)1904561592 Fax +44 (0)1904567621 General Information: info@york-bio.com
                                                                                Copyright © 2004-2006 Yorkshire Bioscience Ltd.