Buffers and solutions for growing cells and for competent cells preparation

 

1M Calcium Chloride Solution

Description: 1M CaCl2, sterile.

Applications: General molecular biology protocols

Shipping Conditions: Ambient

Storage Conditions: Room Temperature

 

Cat.#

Pack Size

Price

 

 

S1003

500 ml

£25

 

 

S1004

2L

£50

 

 

50% Glycerol Solution

Description: 50% Glycerol, sterile

Applications: For use in growth media and for cell stocks dilution

Shipping Conditions: Ambient

Storage Conditions: Room Temperature

 

Cat.#

Pack Size

Price

 

 

S1013

50 ml

£30

 

 

S1014

500 ml

£60

 

 

20% Glucose Solution

Description:  20% Glucose, sterile

Applications: For use in growth media

Shipping Conditions: Ambient

Storage Conditions: +4 ºC (days), -20 ºC (weeks)

 

Cat.#

Pack Size

Price

 

 

S1011

100 ml

£25

 

 

S1012

500 ml

£50

 

 

100mM IPTG Solution

Description: 100mM solution of Isopropyl β-D-thiogalactopyranoside (IPTG)

Applications: Bacteria and cell culture media

Shipping Conditions: On ice

Storage Conditions: +4 ºC (days), -20 ºC (weeks)

 

Cat.#

Pack Size

Price

 

 

S1015

10 ml

£20

 

 

S1016

25 ml

£40

 

 

X-Gal Solution

Description: 40 mg/ml X-gal (5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside) in dimethylformamide

Applications: Blue/White colonies screening test

Shipping Conditions: On ice

Storage Conditions: -20 ºC

 

Cat.#

Pack Size

Price

 

 

S1085

10 ml

£40

 

 

S1086

20 ml

£60

 

 

STM Buffer

Description: 270 mM sucrose, 10 mM Tris-Cl, 1mM MgCl2, pH7.5, sterile

Applications: General molecular biology protocols

Shipping Conditions: Ambient

Storage Conditions: +4 ºC (days), -20 ºC (weeks)

 

Cat.#

Pack Size

Price

 

 

S1062

100 ml

£25

 

 

S1063

1 L

£55

 

 

 

Buffers and solutions for electrophoresis and blotting

 

10x  SDS-PAGE Running Buffer

Description: 0.1 M Tris, 0.2 M Glycine, 0.1% SDS, pH 7.2

Applications:  SDS-PAGE

Shipping Conditions: Ambient

Storage Conditions: Room temperature

 

Cat.#

Pack Size

Price

 

 

S1050

200 ml

£20

 

 

S1051

1 L

£55

 

 

20x SSC Solution

Description: 3M NaCl, 0.3M trisodium citrate, sterile

Applications: DNA and RNA hybridisation protocols

Shipping Conditions: Ambient

Storage Conditions: Room temperature

 

Cat.#

Pack Size

Price

 

 

S1054

500 ml

£35

 

 

S1055

1 L

£55

 

 

20x SSPE Solution

Description: 3M NaCl, 0.2M Na-phosphate (monobasic, anhydrous), 20 mM EDTA, pH 7.4

Applications: DNA and RNA hybridisation protocols

Shipping Conditions: Ambient

Storage Conditions: Room temperature

 

Cat.#

Pack Size

Price

 

 

S1056

500 ml

£35

 

 

S1057

1 L

£55

 

 

10x TAE, electrophoresis grade

Description: 0.4M Tris-acetate, 20mM EDTA, pH 8.0

Applications: DNA electrophoresis

Shipping Conditions: Ambient

Storage Conditions: Room temperature

 

Cat.#

Pack Size

Price

 

 

S1064

1 L

£40

 

 

S1065

4 L

£120

 

 

5x TBE, electrophoresis grade

Description: 450mM Tris, 450mM Boric acid, 10mM EDTA, pH8.0

Applications: DNA electrophoresis

Shipping Conditions: Ambient

Storage Conditions: Room temperature

 

Cat.#

Pack Size

Price

 

 

S1070

1L

£40

 

 

S1071

4L

£120

 

 

10x TBS

Description: 247mM Tris-Cl, 27mM KCl, 1.37mM NaCl, sterile

Applications: DNA and RNA hybridisation protocols

Shipping Conditions: Ambient

Storage Conditions: Room temperature

 

Cat.#

Pack Size

Price

 

 

S1075

200 ml

£20

 

 

S1076

1 L

£70

 

 

 

 

Buffers and solutions for DNA/RNA extraction and purification

 

7.5M Ammonium Acetate (Alkaline Lysis Solution III)

Description:  7.5 M potassium acetate

Applications: DNA purification

Shipping Conditions: Ambient

Storage Conditions: Room Temperature

 

Cat.#

Pack Size

Price

 

 

 S1001

250 ml

£25

 

 

 S1002

1L

£60

 

 

CTAB Extraction Solution

Description: 2% (w/v) CTAB (cetyltrimethylammonium bromide), 0.1 M Tris-Cl, 20 mM EDTA, 1.4M NaCl, pH 8.0, sterile.

Applications:  Plant DNA purification

Shipping Conditions: Ambient

Storage Conditions: Room Temperature

 

Cat.#

Pack Size

Price

 

 

S1005

250 ml

£40

 

 

S1006

1L

£80

 

 

GTE (Alkaline Lysis Solution I)

Description: 50 mM Glucose, 25 mM Tris-Cl, 10 mM EDTA, pH 8.0, sterile.

Applications:  DNA purification

Shipping Conditions: Ambient

Storage Conditions: +4 ºC

 

Cat.#

Pack Size

Price

 

 

S1009

200 ml

£25

 

 

S1010

1 L

£60

 

 

3M KAc (Potassium Acetate, Alkaline Lysis Solution III)

Description:  3M potassium acetate, pH 4.8

Applications: DNA purification

Shipping Conditions: Ambient

Storage Conditions: Room Temperature

 

Cat.#

Pack Size

Price

 

 

S1017

500 ml

£25

 

 

S1018

2L

£80

 

 

6M Lithium Chloride Solution

Description:  6 M LiCl, sterile

Applications: General molecular biology protocols

Shipping Conditions: Ambient

Storage Conditions: Room Temperature

 

Cat.#

Pack Size

Price

 

 

S1019

100 ml

£20

 

 

S1020

500 ml

£80

 

 

 

3M NaAc (Sodium Acetate, Alkaline Lysis Solution III)

Description:  3M potassium acetate, pH 5.2

Applications: DNA purification

Shipping Conditions: Ambient

Storage Conditions: Room Temperature

 

Cat.#

Pack Size

Price

 

 

S1033

500 ml

£35

 

 

S1034

2L

£80

 

 

NaOH/SDS (Alkaline Lysis Solution II)

Description: 0.2 M NaOH, 1% SDS, sterile.

Applications: DNA purification

Shipping Conditions: Ambient

Storage Conditions: Room Temperature

 

Cat.#

Pack Size

Price

 

 

S1035

500 ml

£35

 

 

S1036

2 L

£80

 

 

PEG/NaCl  Solution

Description: 20% PEG 8000, 2.5M NaCl

Applications: DNA purification protocols

Shipping Conditions: Ambient

Storage Conditions: Room temperature

 

Cat.#

Pack Size

Price

 

 

S1037

50 ml

£20

 

 

S1038

500 ml

£100

 

 

STET-E1 Solution

Description: 3M NaCl, 170 mM Tris-Cl, 50mM EDTA, 16.5% Tween-20, pH8.0

Applications: General molecular biology protocols

Shipping Conditions: Ambient

Storage Conditions: Room temperature

 

Cat.#

Pack Size

Price

 

 

S1058

100 ml

£35

 

 

S1059

1 L

£80

 

 

STET Solution

Description: 8% (w/v) sucrose, 0.5% (v/v) Triton X-100, 50 mM EDTA, 50 mM Tris-Cl, pH8.0, sterile

Applications: “Boiling Prep” technique of plasmid DNA purification

Shipping Conditions: Ambient

Storage Conditions: Room temperature

 

Cat.#

Pack Size

Price

 

 

S1060

50 ml

£20

 

 

S1061

500 ml

£100

 

 

 

 

Buffers and solutions for general use

 

0.5M EDTA Solution

Description: 500mM Na2EDTA, pH 8.0

Applications: General molecular biology protocols

Shipping Conditions: Ambient

Storage Conditions: Room Temperature

 

Cat.#

Pack Size

Price

 

 

S1007

100 ml

£20

 

 

S1008

500 ml

£60

 

 

1M Magnesium Sulfate Solution

Description: 1 M MgSO4, sterile

Applications: General molecular biology protocols

Shipping Conditions: Ambient

Storage Conditions: Room Temperature

 

Cat.#

Pack Size

Price

 

 

S1030

100 ml

£20

 

 

S1031

500 ml

£40

 

 

30% PEG Solution

Description: 30% (w/v) polyethylene glycol (PEG 8000)

Applications: General molecular biology protocols

Shipping Conditions: Ambient

Storage Conditions: Room temperature

 

Cat.#

Pack Size

Price

 

 

S1039

50 ml

£20

 

 

S1040

250 ml

£60

 

 

2M Potassium Chloride Solution

Description: 2M KCl, sterile

Applications: General molecular biology protocols

Shipping Conditions: Ambient

Storage Conditions: Room Temperature

 

Cat.#

Pack Size

Price

 

 

S1041

500 ml

£40

 

 

S1042

1 L

£60

 

 

5M Sodium Chloride Solution

Description: 5M NaCl, strile

Applications: General molecular biology protocols

Shipping Conditions: Ambient

Storage Conditions: Room Temperature

 

Cat.#

Pack Size

Price

 

 

S1043

500 ml

£35

 

 

S1044

1 L

£40

 

 

S1045

4 L

£150

 

 

10% SDS Solution

Description: 10% (w/v) sodium dodecyl sulphate (SDS), sterile

Applications: General molecular biology protocols

Shipping Conditions: Ambient

Storage Conditions: Room temperature

 

Cat.#

Pack Size

Price

 

 

S1052

200 ml

£35

 

 

S1053

1 L

£80

 

 

10x TE Buffer

Description: 100mM Tris-Cl, 10mM EDTA, pH8.0, sterile

Applications: General molecular biology protocols

Shipping Conditions: Ambient

Storage Conditions: Room temperature

 

Cat.#

Pack Size

Price

 

 

S1080

50 ml

£20

 

 

S1081

500 ml

£40

 

 


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