Pack size:

Cat.#

Price

100 u

A1005

£100

500 u

A1006

£250

Description:

Verizyme  DNA Polymerase* is a thermostable enzymes isolated from Pyrococcus furiosus. Verizyme   catalyses 5’→3’ synthesis of DNA. The enzyme has 3’→5’ proofreading, and no 5’→3’ exonuclease activities. Verizyme has highest fidelity (lowest error rate) known and is recommended for amplifying DNA that can be used directly for subsequent cloning. Polymerase generates blunt-ended DNA. Properties of Verizyme.

Applications:

• PCR (primer extension, DNA amplification) demanding high fidelity
• Amplifying DNA for subsequent cloning

Features:

• Highest fidelity or lowest error rate of any thermostable DNA polymerases
• Each 500 units of enzyme are supplemented with 0.25ml of  DNA-Softner – universal additive to difficult templates
• Enzyme is supplemented with any 10x PCR buffer containing 15 mM MgSO₄
• Other PCR buffers can be supplied upon request - see the List of PCR buffers

Concentration:

5 u/µl

Unit Definition:

One unit of enzyme catalyses the incorporation of 10 nanomoles of deoxyribonucleotides into an acid-insoluble polynucleotide fraction in 30 minutes at 70 ºC.

Source:

Pyrococcus furiosus Vc1

Activity Assay:

50 mM Tris-Cl (pH8.8 at 25 ºC), 50 mM NaCl, 6 mM MgSO₄, 0.25 mg/ml activated calf thymus DNA, 0.2 mM each of dATP, dCTP, dGTP, and 0.05 mM of [³H] dTTP.

Content:

Enzyme

2ml of 10x Reaction Buffer containing 15 mM MgSO₄

1ml of 50 mM MgCl₂

0.25 ml of DNA-Softner

Storage buffer:

50 mM Tris-Cl (pH 8.0 at 25 ºC), 50 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 1% Triton X-100, 50% glycerol

10xReaction buffer (contains MgSO₄):

100 mM Tris-Cl (pH 8.8 at 25 ºC), 500 mM KCl, 15 mM MgSO₄, 1% Triton X-100

Storage:

  -20 °C  (12 months), +4°C  (4 weeks)

Note: 

• Enzyme is not recommended for use in sequencing reactions
• Optimum temperature for extension  70 ºC
• Allow up to 2 min per 1kb to amplify

Quality Controls:

PCR, activity, endonuclease/nickase

Shipping:

Any temperature equal or below ambient.  

Related Products:

dNTPs

PCR Additives

PCR Buffers

Protocols:

References:

1. Fiala, G. and Stetter, K.O. (1986) Arch.Microbiol. 145, 56

2. Andre, P. et al. (1997) Genome Res. 7, 843-852

*Product use and limitation:

Certain applications of this product are covered by patents issued and applicable in certain countries. Purchase of this product does not include a license to perform any patented applications. Users of this product may be required to obtain a patent license depending upon the particular application and country in which the product is used.

The PCR process is covered by patents issued and applicable in certain countries. Yorkshire Bioscience does not encourage or support the unauthorised or unlicensed use of the PCR process. Use of this product is recommended for persons that either have a license to perform PCR or are not required to obtain a license