Polyclonal Antibody Service
Offer for
20.12.2010-15.03.2011:
2-rabbits service for £200,
2-rabbits service + peptide for £400
Yorkshire BioScience offers a complete service for
production, purification and modification of polyclonal antibodies according to
the instructions of the customer. Our team will precisely determine the degree
of immunogenicity and necessary modifications of the antigen, duration and cost
of a project before immunisation.
Customer provides:
1-5 mg of antigen per rabbit.
You can send us the peptide or we can synthesise it for you.
Standard service:
Polyclonal Antibodies against peptides and proteins
(immunisation, bleeds, IgG precipitation);
Assay of the antiserum titer with ELISA.
Extra services:
Testing the antigen (protein assay, SDS-PAGE);
Peptides synthesis;
Peptide conjugation to a carrier (see
Additional services);
Affinity IgG isolation from antiserum;
Other hosts: mouse, rat, goat,
sheep.
Customer receives:
Total IgG (ammonium sulphate suspension, 33% saturation) or
monospecific IgG;
Sample of preimmune serum;
Complete documentation of the experiments.
Duration: 11-14 weeks
Protocols:
typical immunisation, titer assay, IgG purification, recovery and storage.
Typical immunisation scheme
for standard service:
Day 1: Preimmune bleed. ELISA. First immunisation;
Day 28:First boost immunisation;
Day 42: Second boost immunisation;
Day 52: First bleed 50ml. Third boost immunisation;
Day 62: Second bleed 50ml. ELISA.
Ordering
information
|
Service |
Cat. # |
Price |
|
|
|
£, GBP |
Euro |
|
Polyclonal Antibodies, 1 rabbit |
RPA1 |
350 |
450 |
|
Polyclonal Antibodies, 2 rabbits |
RPA2 |
420 |
570 |
|
Polyclonal Antibodies, 3 rabbits |
RPA3 |
490 |
700 |
|
Extra Immunisation/Bleed inc. terminal |
EIB1 |
95 |
120 |
|
Keeping for Extra month |
KEM |
90 |
115 |
|
Delivery, UK mainland |
SD |
15 |
- |
|
Delivery, EU |
SDE |
- |
60 |
Extras: The animals will be kept for two weeks
for free after the day of antibodies delivery to a customer. Customer then has an option to order another boost(s) and bleed(s) including terminal one (up to 100ml) for an additional charge of £75 per animal.
We can also
keep the rabbits for future bleeds at £60 per animal per month.
