High copy
plasmid purified from 2 ml of E.coli overnight LB-culture using
YORBIO DNA Plasmid Purification Kit (5
ml/lane).
DNA was eluted from spin column with 50
ml
of elution buffer.
DNA marker – Q-Step 4 DNA ladder (Cat# M4042).
Plasmid DNA Purification Kit
Ordering information:
| Product | Pack Size | Cat. # | Price |
| YORBIO Plasmid DNA purification kit | 3 minipreps | D0010 | Free |
| YORBIO Plasmid DNA purification kit | 50 minipreps | D0011 | £45 |
| YORBIO Plasmid DNA purification kit | 250 minipreps | D0012 | £180 |
Related products:
YORBIO Gel/PCR DNA
Purification kit
Protocol:
Q-Step DNA ladders
YORBIO Plasmid DNA Purification Kit (pdf)
Component listing for D0011
| Spin column | 50 |
| 2-ml collection tube | 50 |
| RNase A (5 mg/ml) | 300 µl |
| Solution I, resuspension buffer | 12 ml |
| Solution II, lysis solution | 12 ml |
| Solution III, neutralisation solution | 24 ml |
| Wash solution, concentrate | 17.5 ml |
| Elution buffer, 5 mM Tris-Cl, pH 8.0 | 5 ml |
Electropherogram of plasmid DNA sequenced with M13 forward primer following isolation using YORBIO DNA Plasmid Purification Kit
Description
The kit provides a simple and efficient method for both high and low copy number plasmid DNA purification. Plasmid DNA is selectively absorbed in the spin column, while genomic DNA, proteins, salts and nucleotides are washed off. Up to 25 µg of plasmid DNA from 1-10 ml of overnight E. coli culture can be purified.
Applications
· The Plasmid DNA purification kit has been optimised for efficient
extraction of
plasmid DNA from E. coli.
· The kit is suitable for high and low copy plasmids. The quality of
the purified plasmid
is suitable for all downstream applications (DNA
sequencing, restriction enzyme
digestion, in vitro transcription and PCR).
Features
· Spin column format
· 12 samples
in just 20 minutes
·
More
than 700 bases
can be clearly sequenced
· Reproducible yield up to 25
mg
of plasmid DNA per miniprep
